FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_HBEpC_LOW_12HPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_HBEpC_LOW_12HPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878299
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC168420.8966623524795573No Hit
GTAGTAACAAGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA116100.6181124517449033No Hit
GAGTATGGATGGTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTA64090.34121298046796594No Hit
GCCATATACCGATGGGGTTATCCATGCAACTATCTCCATTCCTACAATGT63150.3362084524348892No Hit
AAATAAAGGTATGTGTGCTTCACAACTAGTTATATTTACAAATAGTGTCA57960.3085770689331145No Hit
GTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATA57670.30703311879525036No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAGTGAGGTTTCAACATTT52770.28094568543133974No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA48460.25799939200308364No Hit
GTAGAAACACGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA43750.23292351217777363No Hit
GCTTTATGGTATGTGTCATGAATACAAATGACAGCCAATTTGGCACCTTA43020.22903701700315018No Hit
GTAATGGTAAGAAATCTTTCGGGTAAGAGTATGGATGGTTCTTCTTTTAC42910.22845138074396037No Hit
GGTCTTAATTGAATCAATCCATTAAAGCATTAGCAGATGCTTTATGGTAT42170.22451164590941058No Hit
TTGTAGAACCATGCTGCAAAGCCATATACCGATGGGGTTATCCATGCAAC41280.2197733161759656No Hit
ATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATATTTGCCC41240.21956035753626021No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA39730.21152116888738162No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT38530.20513240969621985No Hit
GCTTATAGCTTTTTTTGTAAACAGGGAAATGTTGAGTGGTACTTGTATTC35960.19144981709514833No Hit
CCTACAATGTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCC35640.18974614797750516No Hit
CCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATATT34880.1856999338231027No Hit
GTACTTGTATTCCCTTATCAAATTAATCACATAGCTTTTATACAGAAACT33860.1802694885106152No Hit
TTCCTACAATGTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACT33630.17904497633230917No Hit
CTCTAACAGTAAACTTTTGGGAATTTTTGAGCTACATATATGAATCCCTG33050.17595707605658098No Hit
GAAATAGTGATAGGAAATTGCCAACTCTAACAGTAAACTTTTGGGAATTT32660.1738807293194534No Hit
TCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCCCAAAATAAAGG30430.16200828515587773No Hit
GTGTCACTCAGGTCTTAATTGAATCAATCCATTAAAGCATTAGCAGATGC29080.15482093106582073No Hit
ATAGTAGGGACTCCATGGAATATTTGCCCAGTAATGGTAAGAAATCTTTC28990.1543417741264836No Hit
TACAAATAGTGTCACTCAGGTCTTAATTGAATCAATCCATTAAAGCATTA27520.1465155441173104No Hit
CCATGGAATATTTGCCCAGTAATGGTAAGAAATCTTTCGGGTAAGAGTAT27400.1458766681981942No Hit
GGGTTATCCATGCAACTATCTCCATTCCTACAATGTCCTTAATATGAGAT27220.14491835431951994No Hit
GTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTAAACAGGGAAAT26900.1432146852018768No Hit
CATTGATAGTAGGGACTCCATGGAATATTTGCCCAGTAATGGTAAGAAAT26550.14135129710445463No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA26340.1402332642460013No Hit
GCTTTTATACAGAAACTCTTCCTGTGTGAGCTGTTAAGGTGCCAAATTGG24870.13240703423682812No Hit
ACTATCTCCATTCCTACAATGTCCTTAATATGAGATCATCAAACATTGAT24010.12782842348316215No Hit
TTTACAAATAGTGTCACTCAGGTCTTAATTGAATCAATCCATTAAAGCAT22430.11941655721479914No Hit
CTTCACAACTAGTTATATTTACAAATAGTGTCACTCAGGTCTTAATTGAA22320.11883092095560929No Hit
CCATAAAGCATCTGCTAATGCTTTAATGGATTGATTCAATTAAGACCTGA21880.11648837591884999No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA20710.11025933570746724No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG20610.10972693910820375No Hit
CTGAAATAGTGATAGGAAATTGCCAACTCTAACAGTAAACTTTTGGGAAT20410.10866214590967678No Hit
GGGAAATGTTGAGTGGTACTTGTATTCCCTTATCAAATTAATCACATAGC19930.10610664223321208No Hit
CTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATATTT19800.10541452665416955No Hit
CTTTTACCCAATCTGCTTATAGCTTTTTTTGTAAACAGGGAAATGTTGAG19720.10498860937475875No Hit
AACACGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGATAGGA19440.10349789889682101No Hit
TACAAGTACCACTCAACATTTCCCTGTTTACAAAAAAAGCTATAAGCAGA19270.10259282467807308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC11350.0118.838091
TAGTAAC25550.0115.7874762
CACGCAC12100.0113.857213
GCACGCA12450.0110.65642
AAACACG15200.0107.33255
CGCACTT13300.0106.855465
ACGCACT13400.0104.43474
GAAACAC17250.093.736364
AGTAACA36450.082.355983
AACACGT5300.082.085736
GTAACAA36250.081.810234
TAGAAAC20050.081.369272
GCACTTC16300.078.2926
ACACGTG5800.074.99747
AAGAGCC28350.074.926869
GTAGTAA40700.074.298531
AACACGA17000.070.802976
CACGAGC17850.068.2329258
ACAAGAG43750.067.609087
ACACGAG18000.066.858797
Produced by FastQC (version 0.11.5)