FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_HBEpC_LOW_12HP1_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_HBEpC_LOW_12HP1_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398706
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC125680.89854479783457No Hit
GTAGTAACAAGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA94070.6725501999705442No Hit
GCCATATACCGATGGGGTTATCCATGCAACTATCTCCATTCCTACAATGT58420.4176717623288954No Hit
GAGTATGGATGGTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTA51100.3653376763951824No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA49200.3517536923413498No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT47840.34203041954492225No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAGTGAGGTTTCAACATTT40030.2861930956183787No Hit
GTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATA38470.275039929763653No Hit
AAATAAAGGTATGTGTGCTTCACAACTAGTTATATTTACAAATAGTGTCA37550.2684624216954814No Hit
TTGTAGAACCATGCTGCAAAGCCATATACCGATGGGGTTATCCATGCAAC35710.2553074055591382No Hit
GTAGAAACACGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA31920.2282109321043879No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA29870.2135545282568317No Hit
ATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATATTTGCCC28680.20504666455995754No Hit
GGTCTTAATTGAATCAATCCATTAAAGCATTAGCAGATGCTTTATGGTAT28440.20333079288999975No Hit
GTAATGGTAAGAAATCTTTCGGGTAAGAGTATGGATGGTTCTTCTTTTAC27230.19467993988729582No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG26790.19153417515903987No Hit
GCTTTATGGTATGTGTCATGAATACAAATGACAGCCAATTTGGCACCTTA26770.19139118585321002No Hit
CTCTAACAGTAAACTTTTGGGAATTTTTGAGCTACATATATGAATCCCTG25720.18388424729714464No Hit
TCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCCCAAAATAAAGG24350.17408947984780218No Hit
TTCCTACAATGTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACT24260.173446027971568No Hit
GAAATAGTGATAGGAAATTGCCAACTCTAACAGTAAACTTTTGGGAATTT24110.1723736081778444No Hit
GGGTTATCCATGCAACTATCTCCATTCCTACAATGTCCTTAATATGAGAT23560.1684414022675244No Hit
CCTACAATGTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCC21830.15607282731324523No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA21450.1533560305024787No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT21270.15206912675001036No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG21210.1516401588325209No Hit
GCTTATAGCTTTTTTTGTAAACAGGGAAATGTTGAGTGGTACTTGTATTC20800.14870887806300967No Hit
GTACTTGTATTCCCTTATCAAATTAATCACATAGCTTTTATACAGAAACT20610.1473504796576264No Hit
CCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATATT20430.14606357590515806No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA20100.14370425235896606No Hit
ATAGTAGGGACTCCATGGAATATTTGCCCAGTAATGGTAAGAAATCTTTC19270.13777019616702865No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG19230.13748421755536902No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA19160.13698375498496468No Hit
GTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTAAACAGGGAAAT18920.13526788331500686No Hit
GTGTCACTCAGGTCTTAATTGAATCAATCCATTAAAGCATTAGCAGATGC17740.12683151427104766No Hit
CCATGGAATATTTGCCCAGTAATGGTAAGAAATCTTTCGGGTAAGAGTAT17150.12261332974906806No Hit
CATTGATAGTAGGGACTCCATGGAATATTTGCCCAGTAATGGTAAGAAAT16910.12089745807911027No Hit
CTGAAATAGTGATAGGAAATTGCCAACTCTAACAGTAAACTTTTGGGAAT16560.11839514522708847No Hit
TCTATAATCAGCTATGAATAACTGTATGGCTGTTTGTGCTGTTGCTGGAC15880.11353350882887468No Hit
ACTATCTCCATTCCTACAATGTCCTTAATATGAGATCATCAAACATTGAT15850.11331902487012996No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG15780.1128185622997256No Hit
TACAAATAGTGTCACTCAGGTCTTAATTGAATCAATCCATTAAAGCATTA15480.11067372271227835No Hit
CTTTTACCCAATCTGCTTATAGCTTTTTTTGTAAACAGGGAAATGTTGAG15380.10995877618312927No Hit
CTAATAGACCATCTCTTCCTTTAGTGTTTTCCCATAACTCCTTTATGATT15090.10788543124859691No Hit
AACACGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGATAGGA14930.10674151680195838No Hit
CTTCACAACTAGTTATATTTACAAATAGTGTCACTCAGGTCTTAATTGAA14280.10209436436248932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC7600.0129.736011
TAGTAAC18950.0122.809242
GCACGCA8100.0122.622672
CACGCAC8050.0122.483693
CGCACTT8350.0121.556115
ACGCACT8400.0118.243294
GTAACAA21900.0107.590644
AAACACG10100.0100.49445
GAAACAC10500.096.6660464
GCACTTC10350.085.458396
AAGAGCC19100.085.402169
TAGAAAC12200.084.9789662
ACAAGAG28250.083.916827
AGTAACA29450.080.254153
AACACGT3300.079.09046
GTAGTAA32900.072.058811
ACACGTG3800.068.681317
AACACGA11000.068.545016
ACACGAG11450.065.8487557
AACAAGA36400.064.93096
Produced by FastQC (version 0.11.5)