FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_HBEpC_HIGH_6HPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_HBEpC_HIGH_6HPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915595
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27380.2990405146380223No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT19740.21559750763164937No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT18650.20369268071581867No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC17570.19189707239554604No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT13200.14416854613666522No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA12600.13761543040318044No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT12340.1347757469186704No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT11760.12844106837630176No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAGTGAGGTTTCAACATTT11730.12811341258962752No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT11560.12625669646514015No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA11530.12592904067846591No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT11350.12396310595842049No Hit
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC11190.12221560842949121No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG10070.10998312572698629No Hit
GTATAATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTTTTAGTGGAG9310.10168251246457222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA100.007082241145.058351
CTCCGAC151.2298739E-4145.0029145
AGCACGC5850.0128.940771
GTAACAA11400.0127.223314
CACGCAC6050.0127.055093
GCACGCA6200.0123.981182
TAGGACG203.8622864E-4108.7759254
CGCACTT7600.0104.959235
ACGCACT7800.0101.338274
GAAACAC5850.0100.4085544
AAACACG5850.0100.4085545
TAGAAAC6750.091.318062
TAGTAAC16550.089.825042
AACACGT1850.086.236786
TAACAAG17400.083.769975
ACGTGCA2050.081.360862
CGGTGCG554.386675E-879.109772
GTATGCG300.001932602772.5291751
GGTGCGT702.8067006E-972.517293
CATCGGC300.001935536672.50145145
Produced by FastQC (version 0.11.5)