FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_HBEpC_HIGH_12HPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_HBEpC_HIGH_12HPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080005
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT77640.7188855607149967No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT66310.6139786389877825No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT37490.34712802255545117No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT34080.31555409465696915No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT32720.3029615603631465No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG32550.30138749357641864No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT31640.29296160665922844No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT29050.26898023620261013No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT23460.21722121656844182No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA23270.21546196545386365No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA22330.20675830204489792No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC20530.19009171253836787No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT19900.18425840621108236No Hit
AACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTATTCAAT19400.179628798014824No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT18920.175184374146416No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA18020.16685107939315094No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA17920.16592515775389927No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT17920.16592515775389927No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC17120.15851778463988594No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT16720.15481409808287924No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC16120.14925856824736922No Hit
GTTCATAGCTGAGACCATTTGCATTTCTCGTCTCACTCCAGGCACTGAAG16090.14898079175559373No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT15130.1400919440187777No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT14690.13601788880607035No Hit
GTATAATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTTTTAGTGGAG14310.13249938657691399No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT14140.13092531979018615No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT13240.12259202503692111No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT12360.11444391461150642No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA12030.11138837320197592No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA11960.11074022805449976No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC11930.11046245156272425No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGT11890.11009208290702359No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAGGC11880.10999949074309841No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT11830.10953652992347258No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA11820.1094439377595474No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT11780.10907356910384673No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT11560.10703654149749307No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG11480.10629580418609172No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC11450.10601802769431624No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT11410.10564765903861556No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA11400.10555506687469039No Hit
GATCTGGACGTAGCCTTGGGCAGACCAAAATGCACGGGGAAAATACCCTC11020.10203656464553405No Hit
CCCCATAAACATCTTCGAAGCTTATATGTACCCAATCTCACCAAGGTGAG10810.10009212920310555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAAC100.0070904316145.003482
TTCGCAT100.0070904316145.003484
GTAACAA21800.0134.693594
AGCACGC8450.0126.1448061
CACGCAC8650.0125.725553
GCACGCA8750.0124.28872
TAACAAG26000.0112.656545
GAAACAC7350.0112.4516754
AAACACG7750.0106.647715
GAGCAGA1000.0101.5165251
TAGTAAC28850.0101.024952
CGCACTT11600.096.2523045
ACGCACT11850.093.609834
AGTAACA31750.093.167593
TAGAAAC9850.092.743332
AAGCACG950.091.593861
GGAGTGT259.3843334E-487.00611145
ACAGCGT608.2945917E-1084.585364
ACGTGCA3350.080.0765462
TCTACTA1400.077.680435
Produced by FastQC (version 0.11.5)