FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_HBEpC_HIGH_12HPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_HBEpC_HIGH_12HPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504892
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT89810.5967870119583332No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT85380.5673496835653322No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT47000.31231477076095826No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT41890.2783588456846073No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA40870.2715809506595822No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT40310.26785975339094104No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT39730.26400565621984834No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT39080.2596864093901755No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG36820.2446687204131592No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT30030.1995492035308846No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT28230.18758821231025216No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA27760.1844650646026426No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA26620.17688977016290872No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT25570.16991252528420644No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC25420.16891577601582042No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA22920.15230328820938646No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC22810.15157233874590337No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC22610.15024333972138865No Hit
AACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTATTCAAT22380.14871499084319673No Hit
GTTCATAGCTGAGACCATTTGCATTTCTCGTCTCACTCCAGGCACTGAAG22360.14858209094074523No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT22080.14672149230642464No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT22040.1464556925015217No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT21750.14452864391597536No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA21520.14300029503778344No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT20880.13874749815933635No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT20700.13755139903727312No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT20320.13502630089069514No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC19030.12645425718257525No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA18910.1256568577678664No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT18840.12519170810928623No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATC18830.1251252581580605No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT18810.12499235825560905No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC17860.11867961288916415No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAGGC17300.11495841562052292No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA17120.11376231649845969No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC15780.1048580230342111No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT15480.10286452449743903No Hit
CCCCATAAACATCTTCGAAGCTTATATGTACCCAATCTCACCAAGGTGAG15450.1026651746437618No Hit
GTATAATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTTTTAGTGGAG15070.10014007649718384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCG100.007089015145.01505145
CGATAAC100.007089015145.015055
GTAACAA23600.0129.346054
CACGCAC11550.0126.80973
GCACGCA11550.0126.186132
AGCACGC11350.0125.883541
GAAACAC10150.0111.440144
TAACAAG27750.0110.26375
AAACACG11100.0101.9024665
CGCACTT15600.096.6767045
CCCGCAC301.5988988E-596.6767043
ACGCACT15550.095.122414
TAGAAAC12500.092.232642
TAGTAAC33450.090.393552
AACACGT3400.089.568126
CGCACGC259.3738985E-487.0321661
GGTCACC259.383805E-487.00903145
AGACGCG259.383805E-487.00903145
ACAGCGT353.4360724E-582.8657464
AGTAACA37600.081.378133
Produced by FastQC (version 0.11.5)