FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_DVG_TEST_COND6_HighPB2LowDVG_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_DVG_TEST_COND6_HighPB2LowDVG_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125587
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA717066.3705426590747765No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG600885.338370112661216No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA481244.275458049888636No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA334632.9729376760747948No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC288992.567460356240788No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA261512.3233210760252208No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT226862.0154816997708753No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC169261.5037487106727423No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG165131.4670567446141436No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT158241.4058442394945925No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG153651.3650655169258352No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT144371.2826196464600248No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG138821.2333120407396319No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG127681.1343414591675278No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT125731.1170171652657679No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC123261.0950730596568723No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC116481.0348378223984462No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG115581.0268419944437879No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA110240.9794000819128154No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC107880.9584332441650446No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT101500.9017517082197999No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC95190.8456920700043622No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA95150.845336699873044No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC89800.7978059448092418No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA89720.7970952045466054No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA89400.7942522434960603No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA83990.7461884332352808No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG81860.7272649737425895No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC79080.7025667496159782No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG77150.6854201407798776No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA74270.6598334913249708No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG74010.6575235854714029No Hit
GTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTT70050.6223419424709064No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA67820.6025300576499195No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC66870.5940900170311135No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT66860.594001174498284No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA66360.5895590478568071No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC65560.5824516452304442No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA65140.5787202588516036No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA60730.5395407018737778No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCG59150.5255035816867111No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC58200.5170635410679051No Hit
GCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA57340.5094230832445648No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA56950.5059582244642129No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG56530.5022268380853724No Hit
TTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTC54870.4874789776356693No Hit
CCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGG53370.47415259771123863No Hit
CACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA52040.46233654084491027No Hit
TCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCA48280.42893174850100435No Hit
TTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT43420.3857542775458494No Hit
CCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAG42340.37615928400025944No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGT41740.3708287320304872No Hit
AGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCC41270.36665313298749896No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA40890.3632771167399766No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT40380.35874614756567014No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTT39710.3527936978660912No Hit
CAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGA39040.34684124816651224No Hit
GCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACT38830.3449755549770919No Hit
GAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAAT37450.3327152854466159No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGG34070.30268650935023234No Hit
CAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCT33440.29708942978197156No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT32690.29042623981975624No Hit
ACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT32640.28998202715560856No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT31590.2806535612085072No Hit
GAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCC31550.28029819107718906No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA31460.27949860828172324No Hit
GGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCG31240.27754407255947344No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA30660.2723912056553603No Hit
GGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATC30040.266882968619929No Hit
CTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCCAAT28390.25222395070305537No Hit
ATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCAT28150.2500917299151465No Hit
TACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT27740.24644918606913546No Hit
GGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGA27670.24582728833932874No Hit
TCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATAT27520.24449465034688567No Hit
ACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG26510.23552155453110246No Hit
GAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAA26510.23552155453110246No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG25610.2275257265764441No Hit
TTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTAT24470.2173976778338769No Hit
GTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCCAATTAGCA24310.21597619730860432No Hit
CTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTC24200.21499892944747942No Hit
ATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATC24080.21393281905352496No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGG23950.21277786612674096No Hit
CAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCA23740.2109121729373207No Hit
GGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCAC23700.21055680280600256No Hit
TTTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGT23590.20957953494487763No Hit
GAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCA23240.20647004629584387No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG23030.2046043531064236No Hit
AAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAAC22740.202027919654367No Hit
CATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGT22560.20042875406343533No Hit
CCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAG22230.19749695048006063No Hit
GTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTC22040.19580894235629942No Hit
CAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCA22010.1955424147578108No Hit
CCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTT21870.19429861929819728No Hit
CAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATA21280.18905690986125462No Hit
TAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTC20310.18043918417678953No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT19610.17422020687872194No Hit
TTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGC19550.17368715168174473No Hit
AAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC19420.17253219875496076No Hit
GTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTC19130.16995576530290418No Hit
GCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTG17900.15902813376487113No Hit
GAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACC17410.15467484965622383No Hit
AGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTG17380.15440832205773522No Hit
GCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTT16820.14943314021928114No Hit
CAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGC16620.14765628956269042No Hit
GACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAA16550.14703439183288364No Hit
GGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTG16550.14703439183288364No Hit
TCATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCT15850.14081541453481605No Hit
AAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATC15820.14054888693632744No Hit
GTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCC15760.14001583173935023No Hit
CCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCC15570.138327823615589No Hit
TCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGC15480.1375282408201232No Hit
TTTCATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCT15290.135840232696362No Hit
GTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCC15260.13557370509787336No Hit
GCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGA15030.133530326842794No Hit
GGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTC15010.13335264177713493No Hit
GAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAA14820.13166463365337375No Hit
ATTAGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCC14780.1313092635220556No Hit
GCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGG14580.12953241286546485No Hit
CCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCAT14540.1291770427341467No Hit
ATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCC14080.12509028622398802No Hit
CGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAGAA13730.12198079757495423No Hit
CTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAAC13640.1211812147794884No Hit
TTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAA13560.12047047451685208No Hit
CTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCC13170.11700561573650015No Hit
GCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAG13000.11549529267839802No Hit
TTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGC12830.1139849696202959No Hit
GAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAA12460.11069779590560302No Hit
ACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCA12400.11016474070862581No Hit
GTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAA12290.10918747284750091No Hit
CTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAA12270.10900978778184184No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTC12130.1077659923222283No Hit
GTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATA12070.10723293712525109No Hit
GTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTT11680.10376807834489915No Hit
GACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAA11620.10323502314792193No Hit
TCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGC11610.1031461806150924No Hit
CAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGA11510.10225775528679701No Hit
TTAGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCA11400.10128048742567212No Hit
GGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAACGGAA11330.10065858969586536No Hit
GACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGA11270.10012553449888814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAA202.1289634E-6145.02411
GAGTAAA100.007087459145.02411
GTAGCTA100.007087459145.02411
GGGCAAA100.007087459145.02411
GGAATTC100.0070902836145.004763
GCAAACG253.7111022E-8145.004763
GCGTTCC100.0070902836145.00476145
TGTAGGG100.0070902836145.00476145
TTACGCC100.0070902836145.00476145
TAGCTAG100.0070902836145.004762
AGTAAAA100.0070902836145.004762
GTAAAAG100.0070902836145.004763
AAAGGTC100.0070921676144.991888
GAGCAAA76350.0144.64421
GAGCGAA10050.0144.302571
AATTTGT1200.0132.92102145
TTTGTGT600.0132.92102145
AATTGGT2550.0127.945366145
GCGAAGC351.902663E-9124.289793
AGCGAAG351.902663E-9124.289792
Produced by FastQC (version 0.11.5)