FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_DVG_TEST_COND5_LowPB2HighDVG-2_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_DVG_TEST_COND5_LowPB2HighDVG-2_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391251
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG787615.6611639452550255No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA662284.760320028521094No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA558234.012431976688606No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA354722.5496477630564147No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA318522.2894502861094077No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC291722.096817899861348No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT264421.900591625810152No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC250511.8006096671269238No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG221471.5918766635208168No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG216371.5552190079288353No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT195041.401903754247077No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT187041.3444015493969097No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC177581.2764051921615869No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG156661.1260369264783996No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG152461.0958482689320619No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC144671.0398554969592115No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT138830.9978788874185894No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT132990.9559022778779673No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC131880.9479238469550066No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC126140.9066660149750118No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA119930.8620299284600693No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG117240.8426948120792007No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA116930.8404666016412567No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC115530.8304037157924774No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG105190.7560821160236363No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG104540.7514100618795602No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA102970.7401252541777149No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA97990.7043301316584858No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG94550.6796041835729139No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT84820.609667126923898No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC81680.5870975115202073No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG81150.5832879904488838No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC81010.5822817018640059No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCG80970.581994190839755No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA79300.5699906055772825No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC76580.5504398559282258No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA76140.5472772346614666No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA73580.5288765291094131No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA72970.5244919859895878No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA71640.5149322444332475No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA70900.509613290484607No Hit
GCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACT66720.4795683884503946No Hit
GTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTT65620.47166183528349664No Hit
TTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTC65080.46778043645611034No Hit
GCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA63330.4552018291451363No Hit
CCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGG60940.4380230454461488No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA60670.4360823460324557No Hit
CAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGA58970.42386312750179517No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC58050.4172503739440259No Hit
TCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCA57840.41574094106670906No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA55260.3971964800025301No Hit
CACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA53760.38641481659312377No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT52650.378436385670163No Hit
GAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAAT51630.3711048545517667No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGG49930.3588856360211062No Hit
GAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCC49760.35766371416804016No Hit
GAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCA49670.35701681436347577No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA48320.34731331729501No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA47870.3440788182721881No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT47390.34062868598117807No Hit
GGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCG46840.3366754093977291No Hit
CCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAG45570.32754693437776505No Hit
TTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT44060.316693393212296No Hit
GGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATC42910.3084274512650845No Hit
ACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG41940.3014553089270017No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTT41860.3008802868785No Hit
ATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCAT40900.2939800222964799No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGG40570.2916080563464105No Hit
CAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCA39380.28305460337494814No Hit
ACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT37860.2721291844534164No Hit
GGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCAC37570.2700447295275978No Hit
ATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATC37410.26889468543059447No Hit
GGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGA36590.26300070943345233No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGT36530.26256944289707607No Hit
AAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAAC36500.26235380962888794No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT35800.2573223667044983No Hit
TACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT34920.25099712417097997No Hit
CAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCT33740.24251554895558025No Hit
TAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTC33450.24043109402976168No Hit
GAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAA33120.23805912807969232No Hit
AGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCC33000.2371965950069398No Hit
TCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATAT32830.23597467315387374No Hit
CTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTC32730.23525589559324667No Hit
CATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGT31810.22864314203547742No Hit
GTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTC31420.22583990954903177No Hit
CTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCCAAT31010.2228929215504607No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG30050.21599265696844064No Hit
CAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCA29840.21448322409112372No Hit
CCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAG28710.2063610376560376No Hit
CAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATA28590.2054985045832851No Hit
GTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCCAATTAGCA28480.2047078492665953No Hit
AAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC27020.1942136968814398No Hit
GGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTC26900.19335116380868728No Hit
TTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTAT26890.19327928605262457No Hit
GTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTC26340.18932600946917558No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG26330.1892541317131129No Hit
CCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTT26300.18903849844492476No Hit
TTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGC25230.1813475785462149No Hit
TCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGC24930.1791912458643336No Hit
CAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGC24400.17538172479301004No Hit
GAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAA24080.17308163659900336No Hit
GCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGA23690.17027840411255768No Hit
GCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTT23590.1695596265519306No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT23330.16769080489430016No Hit
GGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTG23300.16747517162611206No Hit
CTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAAC22820.164025039335102No Hit
AGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTG22650.16280311748203594No Hit
GAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACC21930.1576279190455209No Hit
CCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCC21850.15705289699701924No Hit
CCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCAT21230.15259647612113125No Hit
TTTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGT21000.15094328773168897No Hit
TCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGC20970.15072765446350084No Hit
TCATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCT20890.15015263241499915No Hit
GGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAACGGAA20790.14943385485437208No Hit
CTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAA20770.14929009934224666No Hit
GACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAA20720.1489307105619331No Hit
GCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTG20480.14720564441642808No Hit
GTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCC20380.146486866855801No Hit
GACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAA19630.14109603515109784No Hit
GTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTT19290.1386521914449657No Hit
GACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGA19240.13829280266465216No Hit
GCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGG18910.13592083671458277No Hit
AAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATC18360.13196756013113378No Hit
GGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCT17630.12672048393855603No Hit
GATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAACGGAAA17340.12463602901273746No Hit
GCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAG17270.1241328847202985No Hit
GTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATA17210.12370161818392224No Hit
CAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGA17120.12305471837935787No Hit
GCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGG17030.12240781857479348No Hit
ATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCC16740.1203233636489749No Hit
GTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCC16730.12025148589291221No Hit
TTTCATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCT16510.11867017525953259No Hit
CAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCAT16180.11629820930946322No Hit
TTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAA16140.11601069828521236No Hit
AATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATC16130.11593882052914967No Hit
CGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAGAA16010.11507628745639717No Hit
CTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCC15890.11421375438364464No Hit
GAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAA15860.11399812111545651No Hit
ATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAG15830.11378248784726838No Hit
TTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGC15350.11033235555625837No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTC15310.11004484453200752No Hit
CGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAA14880.10695410102131103No Hit
ATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTC14270.10256955790148578No Hit
GTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTG14170.10185078034085869No Hit
CATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGT13960.10034134746354181No Hit
GGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACC13930.10012571419535368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7900.0145.043151
GCGCGAA202.1277501E-6145.043141
GAATAGA253.7056452E-8145.043141
GGGCGAA100.0070848535145.043141
GGTCTTT100.0070848535145.043141
AAGGGTC100.0070894235145.011875
GAGGATT100.0070917094144.99623145
TCTGTCG100.0070917094144.99623145
GAGCAAA57800.0143.286561
GCAAAGC850.0136.481753
CTTAGGT950.0129.73347145
AACTGAC750.0125.6634145
TTTGTGT700.0124.282486145
TCAAACT550.0118.646084
AGCAAAA69750.0117.676932
GTAATTT23250.0117.24426145
GTATTTT256.4666056E-6115.99698145
GAGAACT256.4666056E-6115.996987
AATTTGT700.0113.925606145
GAATAAG404.807589E-9108.782361
Produced by FastQC (version 0.11.5)