FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_DARPA_DVG_P2_NEGATIVE_CONTROL.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_DARPA_DVG_P2_NEGATIVE_CONTROL.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGAGGAGGTTATATTGGTAACTGGTTAAATTGGAAAACTGGCAG23.8461538461538463No Hit
GGCTTCCTGCGCCAGATAATTTTGCCGCGCCACATCGATATCGCGTTCTG23.8461538461538463No Hit
AAGTAATTCAAATGTTACCTTCTCTAACAAGATCAGAAACTCGATCACCA23.8461538461538463No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC11.9230769230769231No Hit
CCCTTCCGCCGGTGAACCTGCGGGCCAACCAACCACGACACGCCAACAGC11.9230769230769231No Hit
GGATCAATGATCGGAACTTCTGGAGGGGTGAGAATGGACGAAAAACAAGA11.9230769230769231No Hit
GATGAGTACTCCAGCACGGAGAGGGTAGTGGTGCTCATTGACCGTTGTTT11.9230769230769231No Hit
CCCTGGAACAGGTTGATGACGTTGGTCGGCGTGAGGAGCAGACCGTTCGT11.9230769230769231No Hit
GCACTGGATATGTGCTTCCTGATTTGGTCAACCAGTTCAGTCTACTGAAA11.9230769230769231No Hit
ATATTGTTGAGCGTGTTGAAGAAGAAGTGCGGGTTAATCTGGGCCTTCAG11.9230769230769231No Hit
GCAAAAGCAGGGAGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT11.9230769230769231No Hit
GGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGT11.9230769230769231No Hit
GGTGGGGGCGACGACGCGAGCCGGGCTGCTTCCGGGGCCGTTGCGTGACC11.9230769230769231No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA11.9230769230769231No Hit
GTTTGAAGTTTTCCCGAATAATGCTAGTCCTAACGAATGAGTGATGTGCC11.9230769230769231No Hit
GATCAGGTACGACAGGCCAAAGGTGACCAGCAGCTCGGGCACATGGCCGA11.9230769230769231No Hit
CCTTTGATGGTTGCATACATGTTGGAGAGAGAACTGGTCCGCAAAACGAG11.9230769230769231No Hit
GTCACGCTCAGCAGCGCCAGCACGACTCCCAGCACGCGCAGCCCACGCCC11.9230769230769231No Hit
ATGAACACACCACAAACCATTGACTTGTATACTCTAAATGGTGAATTGAA11.9230769230769231No Hit
ATAGAAATATTTGCAAAAATAGTGCATGAAATAAGCTTTTTCTCTACCAA11.9230769230769231No Hit
GCCACACTGACAGCAACACCTTACACTGGAGCATCCTTCAAAGGCTGGTC11.9230769230769231No Hit
GCCGATTGCGACGAGTCGGCGACCGAGTGGGCGATCTCCATGCCGCGCAG11.9230769230769231No Hit
GAAAGAGACGGGTGAGAGACAATATGACTAAGAAAATGATATCACAGAGA11.9230769230769231No Hit
TCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACG11.9230769230769231No Hit
GCATAACGAGAGTGGTAACCTGAAGGCTCGCGATGCGGCCAGCAACTGTC11.9230769230769231No Hit
GGAAGGTGCTAAAAAGGCCAGAGCTATAGCGATCAAGACTATGGAAGAGG11.9230769230769231No Hit
CTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCATGATGCTG11.9230769230769231No Hit
GATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCG11.9230769230769231No Hit
CTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGG11.9230769230769231No Hit
GGCTGCCAGCCTTTTTGTCACACTGAAGTTGTATGAGATGAGACAGTATT11.9230769230769231No Hit
ACAGTGTCCCCTCCCTTCTTCCTGGCTGCATGGGCATTGCTTCATTGGCT11.9230769230769231No Hit
GGCCTCCCCACAGGGCAACTTATCACATGGCAGTTTGCCTTATCAAAGCA11.9230769230769231No Hit
AGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGACTGTCT11.9230769230769231No Hit
GAATCGCTGCATCTGGACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTT11.9230769230769231No Hit
ATACCGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA11.9230769230769231No Hit
CTCCCACCCGCCGCGATATCGCCGGTGAGCGCCCGCAAATCGGCCAGGCC11.9230769230769231No Hit
CAGTCTTCCTTTTCCCAAACTAAAACTTTGGAGACTGTGGGGAATTTTTC11.9230769230769231No Hit
CTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGA11.9230769230769231No Hit
ATGTTGGGAAGCTGTCATTCCCCGCAATGCTTTCTCTTGTCGTCGGTTCC11.9230769230769231No Hit
AAATACTCAGAGGAAGCAAAATTAAACAGAGGAGAAACTGTCTCTTATAC11.9230769230769231No Hit
ATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGA11.9230769230769231No Hit
GTATAATACTAGCTGTAGATATGTTGTGTATGGTTTTTATCACATTGAGA11.9230769230769231No Hit
GGGGTGCCCGACCCGGAGATGGGCGAACAGGTCCTGTCTCTTATACACAT11.9230769230769231No Hit
GGTTACTTTTGGCCCGTATTTGAGCAGAAAATTATTGAAAATTCTGCGGT11.9230769230769231No Hit
GGATTGGGTGCATTCACAATCAGAGCAAAATCGTCAGAGGATTGACGACC11.9230769230769231No Hit
CCCATACCTCGATGGTGCCTGCGCTGGCGTGGGTGATTGAACATACGGAG11.9230769230769231No Hit
GAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCA11.9230769230769231No Hit
CGTTTGGTTTGCCGAATACCTTGGAAACAGTCTGGTGAAGAAGGGCCGCC11.9230769230769231No Hit
AAAATAGACAATTCTCAAAGAAAAAAAGAACAAAGACAGCTAAGGACGCA11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGAGG50.0145.08
CTGGGAG50.0145.04
GGGAGGA50.0145.06
GTCCTGG50.0145.01
GGAGGAG50.0145.07
TGGGAGG50.0145.05
TCCTGGG50.0145.02
AGGAGGT50.0145.09
CCTGGGA50.0145.03
CACCCCC100.072.5145
Produced by FastQC (version 0.11.5)