FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_A549_LOW_2DPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_A549_LOW_2DPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178342
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT59210.2718122315045112No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT38800.17811711843227557No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT35070.16099400369638928No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA34730.15943318358641573No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG32870.15089457945538395No Hit
GTGCTATACTACAAAAATCCCTGAACCAAATTGGGCTGTCTCTGGTTATT32720.1502059823480427No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT32700.15011416940039718No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT31010.14235597532435218No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA30600.14047380989761937No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT28650.13152204750218285No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT28610.13133842160689185No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG28460.13064982449955057No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27650.12693140011990772No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC27320.12541648648375692No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT25850.11866823483181245No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT25840.11862232835798969No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA25270.11600565935009288No Hit
GTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAA25030.11490390397834684No Hit
ACCTAAAAGAGGTTATTGTTGTGTTCAGTGCCGTTGCAGCAGGTTGGTTT24980.11467437160923308No Hit
GTATTAAAGTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCA24400.1120117961275135No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA22770.10452904089440503No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC22690.10416178910382301No Hit
GTGTAAACACAATAAGTAATGAGGAAACGAGTAACGAGCTACAGAAAACC22440.1030141272582542No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT22320.1024632495723812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCG100.0070894514145.013532
ACCGATC100.0070894514145.013533
CCCGTTA151.2297452E-4145.013533
GCTACCG100.0070894514145.013535
GTAACAA19600.0133.17574
CACGCAC4550.0122.7037663
GCACGCA4800.0116.312942
AGCACGC4850.0113.6371151
ACACGAT203.8652387E-4108.7601553
GAAACAC9900.0106.929194
TAGAAAC11000.0104.8052442
TAGTAAC25150.0102.6338352
AAACACG10800.098.018425
CGCACTT6200.097.065515
ACGCACT6250.091.648564
ACATTCG259.3856157E-487.00613145
AACACGT2900.082.5077066
GAAGCGG3100.077.197041
ACGTGCA5100.075.3501742
AACACGA11150.074.1324846
Produced by FastQC (version 0.11.5)