FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_A549_LOW_2DPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_A549_LOW_2DPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213746
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT34600.2850678807592363No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT24480.20168964511520535No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA22890.18858970493002652No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT21470.17689038728037002No Hit
GTGCTATACTACAAAAATCCCTGAACCAAATTGGGCTGTCTCTGGTTATT20280.16708602953171423No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG19930.1642023948997566No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT18300.15077289647092554No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT17920.14764209315622873No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT17200.1417100447704874No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA16880.13907357882126903No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT16610.13684906067661604No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT16340.13462454253196302No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA16150.13305914087461462No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT15190.12514974302695953No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG15140.12473779522239414No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC14510.11954725288487048No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT14440.11897052595847896No Hit
GTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAA13920.11468626879099911No Hit
GTGTAAACACAATAAGTAATGAGGAAACGAGTAACGAGCTACAGAAAACC13510.11130829679356308No Hit
ACCTAAAAGAGGTTATTGTTGTGTTCAGTGCCGTTGCAGCAGGTTGGTTT13380.11023723250169311No Hit
GTATTAAAGTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCA13350.10999006381895388No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT12860.10595297533421326No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC12730.10488191104234329No Hit
GTATAGATCTGTTTCTTTTGGTTCTCCAACTATGAGTTCCAGACACCGCA12650.1042227945550387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTTG100.00708935145.011613
CGCACAA100.007090223145.00565145
CCCTGCC100.00709197144.993688
GTAACAA12150.0130.689484
CACGCAC2350.0117.243433
GAAACAC5150.0112.630384
GCACGCA2500.0110.2088242
AAACACG5200.0108.7587055
TAGTAAC15350.0104.3894652
AGCACGC2700.0102.0620351
TAGAAAC6350.094.771372
CGGACTA259.384247E-487.006975
ACGTGCA2000.083.381682
CGCACTT3650.081.444885
ACGCACT3950.075.259194
CACGTGC3350.073.600111
GAGCAGA702.8085196E-972.517761
AACACGT1500.072.5058066
CCACAGT300.001935226872.5058066
TTGCGCC502.3060038E-672.50282145
Produced by FastQC (version 0.11.5)