FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_A549_LOW_1DPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_A549_LOW_1DPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581587
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA49630.3137987350680045No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT48040.3037455416616348No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT47880.3027338995578492No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT32680.20662789969821452No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA31210.19733343786968405No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG29630.18734347209480098No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT24550.1552238352996073No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT23060.14580291820810362No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG21940.13872142348160424No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT21920.13859496821863104No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA20930.13233543270145745No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA19960.1262023524472571No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG19750.12487457218603845No Hit
AGATTATACTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA19730.12474811692306524No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG19660.12430552350265903No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT19140.12101768666535574No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA18190.11501106167412858No Hit
GTGCTATACTACAAAAATCCCTGAACCAAATTGGGCTGTCTCTGGTTATT18100.11444201299074916No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG18030.11399941957034294No Hit
GTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAA18030.11399941957034294No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA17840.11279809457209752No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC17560.11102772089047266No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA17400.11001607878668705No Hit
GTATTAAAGTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCA16980.10736051826424978No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT16770.10603273800303113No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT16700.10559014458262492No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT16360.10344040511208047No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT16300.10306103932316085No Hit
GTGTAAACACAATAAGTAATGAGGAAACGAGTAACGAGCTACAGAAAACC16000.1011642103785628No Hit
TCTATAATCAGCTATGAATAACTGTATGGCTGTTTGTGCTGTTGCTGGAC15930.10072161695815658No Hit
CTAATAGACCATCTCTTCCTTTAGTGTTTTCCCATAACTCCTTTATGATT15900.10053193406369679No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGC100.0070878747145.023091
CTCTCGG100.0070878747145.023091
CGGATCT100.007089215145.013925
GGACGTT100.007089215145.013922
ACGTACC151.2296696E-4145.013924
CACGACC100.007091896144.995578
GTAACAA14700.0131.203064
CACGCAC3050.0123.6184163
AGCACGC3100.0119.293191
GCACGCA3250.0116.011132
CTCCGAC256.4649957E-6116.0038145
GAAACAC8500.0113.4520654
TAGAAAC8700.0111.6773762
AAACACG8900.0108.3530965
TAGTAAC18300.0105.3926242
ACGTGCA3550.091.910222
CGCACTT4450.089.615345
GGGTAGC259.3818305E-487.013851
ACGCACT4450.086.3566064
ACGTTCC353.4362605E-582.865094
Produced by FastQC (version 0.11.5)