FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_A549_LOW_12HPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_A549_LOW_12HPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174133
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT32160.27390423401778163No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA30380.2587441116125686No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT29760.2534636195388427No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA22570.1922269453290215No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG21470.18285833035950783No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT18240.15534867003993585No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT16710.1423177783096123No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT16420.1398478707267405No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT16340.13916651691077586No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT15690.13363051715606322No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA15660.1333750094750765No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT15200.12945722503327986No Hit
GTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAA14570.1240915637325584No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA14340.12213267151166009No Hit
GTATTAAAGTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCA14250.12136614846869989No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC14020.11940725624780157No Hit
GTGCTATACTACAAAAATCCCTGAACCAAATTGGGCTGTCTCTGGTTATT13660.11634116407596073No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT13560.11548947180600494No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA13420.11429710262806685No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA13370.11387125649308895No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG13290.11318990267712432No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG13160.11208270272618179No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG13070.11131617968322158No Hit
ACCTAAAAGAGGTTATTGTTGTGTTCAGTGCCGTTGCAGCAGGTTGGTTT12670.10790941060339841No Hit
GTGTAAACACAATAAGTAATGAGGAAACGAGTAACGAGCTACAGAAAACC12350.1051839953395399No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG12290.10467297997756643No Hit
TCTATAATCAGCTATGAATAACTGTATGGCTGTTTGTGCTGTTGCTGGAC12040.10254374930267696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA11350.0131.589114
GAAACAC5250.0114.621844
CACGCAC1900.0114.476473
AAACACG5350.0112.479375
GCACGCA1950.0111.545932
TAGTAAC13850.0107.317662
TAGAAAC5900.0106.913932
AGCACGC2050.0106.118221
CGCACTT2400.090.627215
TCGGCTT259.3862496E-487.002114
ACGCACT2650.084.813394
AACACGT1850.082.29936
GAAGCGG1550.079.531621
AACACGA6150.073.091216
CCCACGC406.661459E-572.50486145
ACACGAG6550.068.624717
ACACGTG2250.067.6654367
AGTAACA22900.065.536533
ACGTGCA2250.064.448762
GAGCATC902.8194336E-1064.4460146
Produced by FastQC (version 0.11.5)