FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_A549_HIGH_6HPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_A549_HIGH_6HPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336211
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA38730.28984943246238803No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT38280.2864817008690993No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC33510.25078374598023817No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT29020.21718126852720118No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA27030.20228841103687967No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT24320.1820071829972961No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT24070.18013622100102455No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA22030.16486917111144872No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG21950.16427046327264183No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT21820.16329756303458062No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA19450.14556084330992633No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG18790.14062150363976947No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT17230.12894670078303502No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT16940.12677638486736004No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA16580.12408219959272898No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG16230.12146285279794881No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT16200.12123833735839624No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG14900.1115093349777842No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT14510.10859063426360059No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT13650.10215452499642645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA100.007087029145.028031
TACCCTA100.0070902016145.006332
GTAACAA16900.0120.119094
CACGCAC4000.0112.37573
GAGCCGC203.8662486E-4108.75068145
AGCACGC4050.0107.428181
GCACGCA4200.0107.028472
GAAACAC4800.099.688114
ACGTGCA2300.097.721662
AAACACG5100.093.824115
TAGTAAC23050.088.073252
TAGAAAC5800.085.003712
CGCACTT5600.084.1523065
CACGTGC4150.082.124311
ACGCACT6050.077.893044
ATGCCGG502.3065804E-672.50046145
GAAGCGG1600.067.9818951
TAACAAG31950.063.5371675
AGTAACA32800.062.553743
AACACGA6500.062.4619266
Produced by FastQC (version 0.11.5)