FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_A549_HIGH_1DPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_A549_HIGH_1DPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350035
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT66870.49532049169095616No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT41910.3104363960934346No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT32020.2371790360990641No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG26860.19895780479765338No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT25590.18955064127967053No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT24850.1840693019069876No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT24490.18140270437433104No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA22020.16310688241415963No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC21930.1624402330309955No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT20660.15303306951301263No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA20030.14836652383086363No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC19310.14303332876555053No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT18960.14044080338657886No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT18600.1377742058539223No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT18560.1374779172391827No Hit
AACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTATTCAAT17990.13325580447914315No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA17960.1330335880180884No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT17580.13021884617806206No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA17070.12644116634013192No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG15340.11362668375264344No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC15330.11355261159895855No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA14310.10599725192309828No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT14020.10384915946623607No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT13890.10288622146833229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGC100.0070867282145.030151
CCGAATT100.0070898687145.008674
TGTAGCG100.0070906538145.0033145
GGTTAGC100.0070906538145.0033145
GTAACAA18550.0130.546894
AGCACGC6250.0128.786791
CACGCAC6600.0124.136213
GCACGCA6600.0124.136212
TAGAAAC6900.0111.383462
GAAACAC6600.0109.855054
CGCACTT7800.0108.75655
AAACACG6850.0105.845745
ACGCACT8150.0102.306744
TCTACTA650.0100.390615
TAGTAAC24700.099.216452
ACGTGCA2800.098.398742
TAACAAG27100.089.359595
CTGTCCG259.386617E-487.001976145
AGTAACA31950.076.9294743
AAGCACG702.8085196E-972.515081
Produced by FastQC (version 0.11.5)