FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_A549_HIGH_12HPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_A549_HIGH_12HPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531674
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT69210.4518585547577356No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT64240.4194103967293301No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT44630.29138054181242223No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT39310.25664730223272053No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT36490.23823607373370573No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT33470.218519084348236No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA31250.20402513850858603No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG28890.1886171600484176No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA27590.18012971428646044No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT25530.16668037715597445No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT25520.16661508911165168No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT24280.158519371615631No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC24250.15832350748266277No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT21860.14271966488952612No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA21700.14167505618036214No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA21650.14134861595874842No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC21360.13945526267338873No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT20820.13592970827996034No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC20480.13370991477298694No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT19410.12672409403045296No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT18820.12287209941541084No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA18380.11999942546520996No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT18370.11993413742088721No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT18280.11934654502198248No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT18190.11875895262307777No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA17850.11653915911610435No Hit
GTTCATAGCTGAGACCATTTGCATTTCTCGTCTCACTCCAGGCACTGAAG17800.1162127188944906No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC17480.11412350147616268No Hit
AACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTATTCAAT17470.11405821343183993No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT16660.10876988184169739No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT16450.10739883291091969No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAGGC15920.10393856656181408No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT15560.10158819696619516No Hit
GTATAATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTTTTAGTGGAG15520.10132704478890417No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC15400.1005435882570312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA202.130324E-6145.008872
CACGCAC8700.0133.34153
GCACGCA8850.0131.900732
GTAACAA19900.0131.89254
AGCACGC8800.0131.847781
GAAACAC7900.0107.384
CGCACTT11800.099.539995
ACGCACT11950.098.290544
TAGTAAC27000.097.4781952
AAACACG8900.095.314835
TAACAAG28050.094.087755
TAGAAAC10450.087.4216162
ACGTGCA2600.080.870332
AGTAACA33600.078.546483
AAGCACG651.5625119E-978.094451
GAAGCGG2050.074.2849661
AACACGT3150.071.353586
GCACTTT19000.066.39886
TCTACTA902.8194336E-1064.448395
GACGACA1250.063.79766145
Produced by FastQC (version 0.11.5)