FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah9120spikeb-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah9120spikeb-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555220
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC66010.42444155810753464TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC51730.33262175126348686TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA67450.048.9842769
AGAGCAC70600.046.9318548
AAGAGCA82100.040.7599957
GAAGAGC83550.039.787976
CGGAAGA83950.039.4303634
TCGGAAG84950.038.69073
CACACAG42200.038.42288230-31
GTCACAC42900.037.96026628-29
ACACAGT42850.037.8948832-33
CACAGTG42500.037.65403432-33
GGAAGAG89150.037.394155
CAGTCAC45300.036.62349326-27
ATCGGAA91000.036.221752
GTATGCC22350.036.0636744-45
CGTATGC22050.035.7017544-45
TATGCCG20750.035.67350446-47
CCAGTCA46200.035.6557326-27
AGTCACA46800.035.600328-29
TCCAGTC46000.035.45316324-25
TCACACA47600.034.9032430-31
Produced by FastQC (version 0.11.2)