FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah9120spikea-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah9120spikea-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9354160
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT2765152.9560644675737855TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTAT157160.16801081016360636TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT135680.14504776484473217TruSeq Adapter, Index 16 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA763150.054.706589
AGAGCAC790650.052.9048588
AAGAGCA864700.048.570197
GAAGAGC861100.048.566876
CGGAAGA880450.047.5849274
TCGGAAG885900.047.0377243
GGAAGAG895500.046.6959575
ATCGGAA919350.045.3723072
GATCGGA892850.044.569731
GCCGTCT387150.043.71532450-51
TGCCGTC416350.043.3924950-51
CCCGATC371500.043.15949238-39
TATGCCG433300.042.866548-49
GTCCCGA428000.042.78787636-37
CGATCTC365150.042.7516440-41
GATCTCG366850.042.7136740-41
GTATGCC437850.042.63036346-47
ATGCCGT431750.042.49243548-49
CCGATCT366350.042.4640738-39
CGTATGC441200.042.4185246-47
Produced by FastQC (version 0.11.2)