FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah9048spikeb-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah9048spikeb-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5567788
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC527830.948006641057454TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA127600.053.7393959
AGAGCAC133450.051.3484238
TCGGAAG148750.046.2705763
CGGAAGA154650.044.9005174
GATCGGA142000.044.6742821
ATCGGAA159250.043.190252
GAAGAGC162550.042.110976
GGAAGAG173850.039.6171955
AAGAGCA174200.039.5525057
TGCCGTC75200.038.74913848-49
GCCGTCT70900.038.18250748-49
ACTGACC88400.037.5087832-33
GTATGCC82250.037.4563144-45
TATGCCG81450.036.9875146-47
ATGCCGT80850.036.79695546-47
CGTATGC84550.036.6875344-45
CTCGTAT82000.035.27787442-43
CAGTCAC98500.034.76014326-27
CCAGTCA99900.034.4847226-27
CTGACCA95300.034.47247332-33
Produced by FastQC (version 0.11.2)