FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah8584spikeb-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah8584spikeb-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39722447
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC550010.1384632724162235TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1238350.033.671849
AGAGCAC1337200.031.2038028
CAGTCAC679700.030.46603826-27
AAGGGGG319400.030.27566572-73
AGTCACC691650.029.8139528-29
CCAGATC694700.029.14520632-33
CCAGTCA705900.029.13219826-27
TCCAGTC712450.028.86436724-25
CAGATCA703450.028.52879134-35
CATCTCG262700.028.36579138-39
CATCGCG176500.028.01300838-39
GAACTCC767600.026.94361320-21
GTCACCA774100.026.8236228-29
ACTCCAG774300.026.7165422-23
CTCCAGT774600.026.50899724-25
TCACCAG777700.026.48793630-31
GATCATC675200.026.36037836-37
CACCAGA806550.025.77647230-31
GAAGAGC1643000.025.4635336
ACCAGAT810350.025.43810332-33
Produced by FastQC (version 0.11.2)