FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah8584spikea-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah8584spikea-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5884115
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT617501.0494356415535726TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT92300.039.13178350-51
TGCCGTC97550.038.3266150-51
TATGCCG102650.037.08630448-49
ATGCCGT103050.036.645948-49
GATCTCG94500.036.50494840-41
CGATCTC94800.036.4885840-41
GTATGCC104450.036.37969646-47
CGTATGC105950.035.97554846-47
CTCGTAT101950.035.58914644-45
CCGTCTT104800.035.06977552-53
ATCTCGT103350.033.90194742-43
TCGTATG112350.033.40329444-45
TCTCGTA106750.033.2844542-43
AGGGGGG67300.032.57797274-75
ACGATCT107850.032.1387838-39
CTGCTTG103700.031.92932558-59
AACGATC112550.031.2560338-39
CACGTGA129200.030.66567830-31
TCACGTG131150.030.084330-31
GTCACGT133150.029.75595528-29
Produced by FastQC (version 0.11.2)