FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah8583spikeb-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah8583spikeb-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20164242
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1525340.7564578921439249TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC316700.15706020588326602TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA907100.038.1027689
GCCGTCT211600.037.28153248-49
TGCCGTC242550.037.03915448-49
GTATGCC293400.036.61079444-45
TATGCCG286450.036.235746-47
CGTATGC296350.036.11155344-45
AGAGCAC962100.035.953878
ATGCCGT264950.035.46872346-47
CTCGTAT273550.033.9584742-43
CAGTCAC504800.033.73160626-27
CCTTGTA487150.033.63232-33
GTCACCT499550.033.502828-29
ATCTCGT298300.033.4412540-41
TCGTATG313650.033.42296642-43
AAGGGGG237500.033.39398272-73
AGTCACC510000.033.3646428-29
CCAGTCA522650.032.6245426-27
TCTCGTA296900.032.48293340-41
CCGTCTT264000.032.1693550-51
CACCTTG514200.032.14153730-31
Produced by FastQC (version 0.11.2)