FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah8583spikea-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah8583spikea-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457541
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3000.026.63277650-51
GCCGTCT3300.025.63582650-51
ATGCCGT3350.025.25320248-49
TATGCCG3350.024.55172348-49
GTATGCC3650.023.82141946-47
ATCGCGT802.0384177E-623.4995142-43
CGTATGC3750.022.55952846-47
AGGGGGG3450.022.47778974-75
AAGGGGG3500.020.8138574-75
CAGTCAC6500.020.6072626-27
CACGTCC1059.5253927E-720.14243530-31
CCGTCTT4500.019.84402852-53
GCTTGAA4150.019.81886360-61
ACGTCCG959.03951E-619.7890632-33
GAGCACA17450.019.661489
AGAGCAC17900.018.9046338
ACTCCAG8550.018.68966922-23
CTGCTTG3550.018.53482258-59
ATCTCGT4550.018.07654442-43
AGTCACG4300.018.03450628-29
Produced by FastQC (version 0.11.2)