FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah8104spikeb-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah8104spikeb-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13192509
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2519531.909818670580403TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA711150.047.5837949
AGAGCAC755900.044.828968
GCCGTCT277750.041.88021548-49
TGCCGTC301950.041.7145548-49
GTATGCC336750.041.0882844-45
TATCTCG364400.040.93057638-39
ATGCCGT320100.040.47984746-47
CGTATGC340100.040.4348244-45
TATGCCG338800.040.22929446-47
CTCGTAT348800.039.9180842-43
GTCACGC413950.039.68708828-29
TCACGCC411650.039.66335730-31
CACGCCA414500.039.4586730-31
ATCTCGT374500.039.3623740-41
AAGAGCA870050.039.1689077
TCGTATG347950.039.13086342-43
GAAGAGC871350.038.8802766
AGTCACG429550.038.84207528-29
TCTCGTA372500.038.50756540-41
CCGTCTT315350.038.27277450-51
Produced by FastQC (version 0.11.2)