FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah8104spikea-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah8104spikea-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14844980
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2121661.429210413217128TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA717350.042.7043389
AGAGCAC749850.040.916138
GCCGTCT262550.039.6954250-51
TGCCGTC297300.038.96033550-51
CATCTCG316600.038.95306840-41
GTATGCC319500.038.9157746-47
CCGCACA365900.038.38017736-37
TATGCCG320100.038.14540548-49
CGTATGC320600.037.92465646-47
CGTCCGC384500.037.9231332-33
ATGCCGT317050.037.90458748-49
GTCCGCA381850.037.6693734-35
ACGTCCG387500.037.47792432-33
CTCGTAT320100.037.41861744-45
TCCGCAC389050.037.2863334-35
GCACATC363850.037.05927738-39
CGCACAT375950.036.87288336-37
ATCTCGT332150.036.54928642-43
TCTCGTA337950.036.29055442-43
TCGTATG330900.036.23995244-45
Produced by FastQC (version 0.11.2)