FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah7797spikeb-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah7797spikeb-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3512701
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT659351.8770456124788302TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA121100.069.430789
AGAGCAC126900.066.4426048
TCGGAAG142700.058.812143
CGGAAGA144100.058.4038934
GAAGAGC147800.057.1326876
GATCGGA139650.056.2753451
ATCGGAA149700.056.0934872
AAGAGCA155050.054.7737167
GGAAGAG154750.054.506035
TGCCGTC80350.041.58814650-51
TATGCCG86700.040.98156448-49
CGTATGC87200.040.9621746-47
GCCGTCT73550.040.67256550-51
GTATGCC88350.040.53537846-47
CAGTCAC103500.040.3235226-27
CACAGTC103800.040.0711430-31
CTCGTAT88100.039.69016344-45
ATGCCGT86150.039.66111848-49
GTCACAG105600.039.58839428-29
GAACTCC105200.039.56021520-21
Produced by FastQC (version 0.11.2)