FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah7797spikea-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah7797spikea-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17570558
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG138800.022.19583732-33
GTTTCGG142750.021.69689434-35
AGTCACG150650.021.27666328-29
ATCGGGG50600.020.3878942-43
GTCACGT155000.020.37632628-29
CGGAATC148700.019.56446538-39
AATCGGG69600.018.46870240-41
GGAATCG116150.017.9053338-39
CAGTCAC183900.017.67253126-27
TCGGAAT176550.017.59632936-37
GAATCGG81200.017.07474540-41
CCAGTCA193850.016.93514426-27
GAGCACA388200.016.7922639
TCGGGGG25750.016.60934442-43
CACGTTT192950.016.47825830-31
AAGGGGG75600.016.47450474-75
TCACGTT193900.016.40964330-31
TCCAGTC196600.016.38748224-25
TTTCGGA194050.016.13053934-35
ACGTTTC196850.016.11597632-33
Produced by FastQC (version 0.11.2)