FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah119spikeb-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah119spikeb-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43470900
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4874551.1213363422427416TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC1720960.3958878238085708TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC991540.22809281611376805TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGC667930.1536499129302591TruSeq Adapter, Index 2 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC599570.1379244506094882TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGG511310.11762121327140686TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGC474040.10904766176913752TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA3410750.068.972699
AGAGCAC3505050.067.192148
CGGAAGA3758600.062.5860634
GAAGAGC3777100.062.3604286
TCGGAAG3767300.062.0312463
AAGAGCA3837200.061.6650127
GGAAGAG3899200.060.320855
ATCGGAA3888800.060.2517472
GATCGGA3663900.059.4063761
CCGATGT2625000.043.3196132-33
CGATGTA2654300.042.6687834-35
CACCGAT2722900.042.31701730-31
ACCGATG2697150.042.31588432-33
GTCACCG2751700.041.8860728-29
TATCTCG1218500.041.7648638-39
CAGTCAC2800750.041.66013326-27
TATCGCG684700.041.6071638-39
AGTCACC2797200.041.38788228-29
TCACCGA2785650.041.36799230-31
ATGTATC2609200.041.133136-37
Produced by FastQC (version 0.11.2)