FastQCFastQC Report
Fri 13 Oct 2017
HNKY2AFXX_n01_ah119spikea-100917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKY2AFXX_n01_ah119spikea-100917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10159916
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1022591.0064945418840077TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT247570.24367327446408021TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA564700.046.599529
AGAGCAC590800.044.6204268
CCGTATC295800.040.0556238-39
GAAGAGC670650.039.3047686
AAGAGCA676050.039.2371067
GTTCCGT319650.038.71372234-35
TATCTCG180150.038.25926240-41
CGGAAGA688900.038.1270454
AGTTCCG331900.037.68842334-35
TCGGAAG692000.037.6782573
CGTATCT223850.037.33046738-39
TCCGTAT325550.037.23973536-37
GGAAGAG704850.037.1975675
GTATGCC154500.036.85392446-47
TTCCGTA336950.036.69814736-37
TGCCGTC126350.036.32333850-51
TATGCCG151400.036.1960748-49
CAGTTCC348050.036.02065332-33
GTCACAG360150.035.97189728-29
ATCGGAA728000.035.8667072
Produced by FastQC (version 0.11.2)