FastQCFastQC Report
Tue 6 Oct 2020
HNKW7DRXX_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKW7DRXX_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74511721
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4507270.6049075151545621No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1346330.1806870089606439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC484900.052.4086271
GATCACG180350.049.34841
GATCCAC261450.047.5292781
GATCCTG212600.045.2143171
GATCATG334000.045.129511
GATCGCG83000.042.4008981
GATCGTG208000.042.019311
GATCCCG118200.041.2774471
GATCCGA244450.041.2269331
GATCCAA516550.040.9063841
GATCACA479500.040.761341
GATCCGG140650.040.1171231
GATCCAG257950.039.9431341
GATCCTA190250.039.9009861
GATCCAT423900.039.7095871
GATCGTC259800.039.3508381
GATCCCA198650.039.275251
GATCGCA160350.037.9092451
GATCCTT444250.037.0558471
GATCGTT330700.036.4330981
Produced by FastQC (version 0.11.5)