FastQCFastQC Report
Tue 6 Oct 2020
HNKW7DRXX_n02_JK10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKW7DRXX_n02_JK10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549535554
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC8619150.056.3833921
GATCACG3440500.052.4394721
GATCCAC5107050.048.838021
GATCATG6177850.046.8948671
GATCGCG1411300.046.474451
GATCGTG3905650.045.1201251
GATCCTG4058200.044.403991
GATCCGA4775450.043.6522871
GATCACA8533650.043.1768261
GATCCAA9394400.042.479161
GATCCCG2307500.042.2498251
GATCCCA3917050.041.6558231
GATCCAT8100900.041.244241
GATCCAG5078550.041.1670461
GATCGCA3036800.041.0575941
GATCCGG2724000.040.5079771
GATCCTA3449250.040.3664931
GATCGTC5023150.040.2384761
GATCCGC2107550.037.5878031
GATCGTT6294250.037.11751
Produced by FastQC (version 0.11.5)