FastQCFastQC Report
Tue 6 Oct 2020
HNKW7DRXX_n02_JK09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKW7DRXX_n02_JK09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537695126
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC8478450.055.9727861
GATCACG3368700.051.9178621
GATCCAC4909100.047.939531
GATCATG6185000.046.55681
GATCGCG1376250.046.5507971
GATCGTG3854750.045.2246321
GATCCTG3978600.044.0630761
GATCCGA4611450.043.2864571
GATCACA8445350.042.8068541
GATCGCA2998300.041.7470471
GATCCCA3751550.041.504971
GATCCAA9138400.041.4726751
GATCCGG2613600.041.4444541
GATCCCG2214850.041.2780721
GATCCAG4909850.040.286171
GATCCAT7799650.040.135331
GATCCTA3386050.040.0955431
GATCGTC5010350.039.9111671
GATCGTT6224350.037.2510871
GATCCGC2012150.036.520751
Produced by FastQC (version 0.11.5)