FastQCFastQC Report
Tue 6 Oct 2020
HNKW7DRXX_n02_JK08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKW7DRXX_n02_JK08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489200134
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5589130.11425037753566927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC7864050.059.0456921
GATCACG3122450.054.3530041
GATCCAC4708550.051.0187871
GATCATG5681450.049.2564851
GATCGCG1287150.048.81481
GATCGTG3554450.046.850751
GATCCGA4277050.045.4492381
GATCCTG3687350.045.1483761
GATCACA7776600.044.876511
GATCCAA8489350.043.907531
GATCGCA2750550.043.631511
GATCCCG2102900.043.344511
GATCCCA3568800.043.1627431
GATCCAG4635900.043.0707781
GATCCAT7363750.042.7635461
GATCCGG2475450.042.5203361
GATCCTA3123900.041.8945961
GATCGTC4725300.040.8199351
GATCATT12644550.038.676641
GATCCGC1927000.038.3379751
Produced by FastQC (version 0.11.5)