FastQCFastQC Report
Tue 6 Oct 2020
HNKW7DRXX_n02_JK07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKW7DRXX_n02_JK07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514390734
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6729900.13083245002620908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC8372500.063.2287451
GATCACG3295900.058.1553271
GATCATG6008700.055.0365561
GATCCAC4724700.053.291131
GATCGTG3764400.053.0169751
GATCGCG1340750.052.124361
GATCCTG3811600.050.2032851
GATCCGA4479300.048.3919141
GATCACA8041100.048.3477821
GATCGCA2855150.047.546031
GATCCAA8851250.046.97911
GATCCAT7525050.046.717961
GATCCCG2120950.046.5306171
GATCCCA3635150.046.509311
GATCCTA3277000.046.1158261
GATCGTC4867850.046.048431
GATCCAG4718550.045.353811
GATCCGG2531350.044.9613341
GATCGTT5971600.044.4107061
GATCATT13848950.043.716651
Produced by FastQC (version 0.11.5)