FastQCFastQC Report
Tue 6 Oct 2020
HNKW7DRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKW7DRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74511721
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA829460.11131939899764226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC496250.055.4716341
GATCACG187100.053.0793341
GATCATG320400.049.024411
GATCCAC282500.048.8883131
GATCCAG264850.045.532361
GATCCGA242500.045.4007031
GATCGTG199550.045.2697371
GATCCCG129300.044.9533581
GATCCTG208600.044.5896381
GATCCCA220600.044.034411
GATCACA499100.043.2914281
GATCCAA530100.042.5622061
GATCGTC267550.042.3944661
GATCGCA161750.042.0657581
GATCCGG140850.042.0379561
GATCCTA191450.040.077041
GATCCAT451100.039.2490121
GATCCTT462700.039.0941621
GATCGTT332250.038.64861
GATCATC660800.037.7586441
Produced by FastQC (version 0.11.5)