FastQCFastQC Report
Tue 6 Oct 2020
HNKW7DRXX_n01_JK10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKW7DRXX_n01_JK10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549535554
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC8661850.057.076831
GATCACG3335700.053.1988071
GATCCAC5226200.049.522871
GATCATG5908400.047.421771
GATCGCG1358450.046.9272961
GATCGTG3692800.046.015371
GATCCTG3970100.045.0798071
GATCCGA4592650.044.334591
GATCACA8718200.043.802731
GATCCAA9447700.042.654081
GATCCCG2366750.042.6521761
GATCGCA3013550.041.8408471
GATCCAG4934050.041.8285831
GATCCAT8247750.041.559761
GATCCGG2542750.041.5428731
GATCCCA4061800.041.5171471
GATCGTC5019350.041.23481
GATCCTA3523850.040.4134831
GATCATC11484950.037.8455281
GATCCTT8916750.037.4743581
Produced by FastQC (version 0.11.5)