FastQCFastQC Report
Tue 6 Oct 2020
HNKW7DRXX_n01_JK09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKW7DRXX_n01_JK09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537695126
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC8625950.056.5060731
GATCACG3283900.052.8759081
GATCCAC5051950.048.1867941
GATCATG5844450.047.4862631
GATCGCG1355650.046.842171
GATCGTG3645200.046.4828071
GATCCTG3863900.044.152221
GATCCGA4421800.043.775381
GATCACA8646200.043.2528881
GATCGCA2949000.042.367931
GATCCCG2270200.042.1270561
GATCCAA9149400.041.7771071
GATCCCA3974550.041.54691
GATCCAG4729450.041.205411
GATCCGG2425700.041.1101341
GATCGTC5026700.040.6866381
GATCCAT7966500.040.429951
GATCCTA3491350.040.1828881
GATCGTT6170600.037.181111
GATCCGC2042050.036.972511
Produced by FastQC (version 0.11.5)