FastQCFastQC Report
Tue 6 Oct 2020
HNKW7DRXX_n01_JK08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKW7DRXX_n01_JK08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489200134
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC7942650.059.232371
GATCACG3038250.054.9922331
GATCCAC4840450.051.0621341
GATCATG5426500.049.5498051
GATCGCG1240050.048.856871
GATCGTG3380200.048.0084081
GATCCTG3595200.046.3379331
GATCCGA4154000.046.2024351
GATCACA7928800.045.2740331
GATCCAA8505650.044.4815031
GATCCCG2147000.044.006821
GATCCAG4565650.043.5683251
GATCGCA2744850.043.5313381
GATCCCA3731550.043.418121
GATCCGG2333250.043.3107151
GATCCAT7464100.043.114921
GATCCTA3198200.041.5998421
GATCGTC4677550.041.390861
GATCCGC1932950.038.7550131
GATCGTT5572750.038.524571
Produced by FastQC (version 0.11.5)