FastQCFastQC Report
Tue 6 Oct 2020
HNKW7DRXX_n01_JK07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKW7DRXX_n01_JK07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514390734
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC8424450.063.6844941
GATCACG3198050.059.5849571
GATCATG5764950.055.0581
GATCGTG3537100.054.2056541
GATCGCG1269450.053.7372361
GATCCAC4854600.053.6841161
GATCCTG3750750.050.325051
GATCCGA4299600.049.4837231
GATCACA8326800.048.662381
GATCGCA2833850.048.1288571
GATCGTC4824500.047.3251231
GATCCAA8926650.047.0907671
GATCCTA3372700.046.9627841
GATCCCA3813450.046.83071
GATCCCG2200900.046.736291
GATCCAT7671400.046.34441
GATCCAG4559850.046.2633551
GATCCGG2365050.045.400271
GATCGTT5923500.044.96791
GATCCTT8443450.043.355951
Produced by FastQC (version 0.11.5)