FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80634722
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG102700.064.520775
CTATACG71750.064.442086
TATAGGG219400.063.853987
GTATAGG202800.063.348916
GTATAGC99650.063.233916
GCTATAC73350.063.226985
CGTATAA89750.062.2262955
TATACGG124250.062.0999457
GGTATAC96650.062.0145875
CTTATAG89200.061.826375
TATAGGC148300.061.7845767
TTATACG101300.061.7554366
TATAAGG144650.061.7008257
CTATAGG111700.061.51236
CGTATAC73550.061.1068535
AGTATAG147300.060.8103455
GTATACG102450.060.686996
TGTATAG158650.060.6453825
TATAGTC138450.060.5263867
GTATAGA115650.060.136636
Produced by FastQC (version 0.11.5)