FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_root_plusN_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_root_plusN_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4095926
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA74660.18227868374575126No Hit
GTTTTCTATATCGCCTGCGTAGTGATTACGTGGCTGGTATATGGCCGTAA68180.16645808542439489No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA55640.13584229793214037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG60300.068.315046
GTATAGC59150.068.045646
CGTATAG56150.067.816665
GCTATAG57150.067.732345
CTATAGG74050.067.6823046
CATATAG64200.067.6540765
CTTATAG54500.067.622175
CCTATAC33400.067.5881355
GGTATAC60550.067.570575
GTATAGG128150.067.5675666
GATATAC47500.067.493065
TTATAGC63150.067.448846
GGTATAG93300.067.40995
CTTATAC45450.067.3030555
CTATAGC46950.067.165526
GTATACG57600.067.142426
GCTATAC46000.067.1070255
AGTATAC47450.067.047855
AGTATAG80900.066.96995
GTTATAC47000.066.945145
Produced by FastQC (version 0.11.5)