FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_root_plusN_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_root_plusN_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4599768
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA82480.17931339145800396No Hit
GTTTTCTATATCGCCTGCGTAGTGATTACGTGGCTGGTATATGGCCGTAA71130.15463823392831985No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA61870.13450678382040138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC69600.068.538826
CTTATAG58800.068.5090565
TTATAGC71500.068.283876
GCTATAC50600.068.267915
GGTATAC62750.067.9892045
CTATAGG81850.067.901886
GCTATAG63800.067.857685
TTATACG66550.067.683136
CCTATAC37300.067.6513445
CTATAAG55650.067.544366
AGTATAC55500.067.537735
CTATACC42150.067.506096
GTATAAG87900.067.409036
CTATACG48600.067.40466
CGTATAA59800.067.363425
CTATAGC50400.067.288876
CTTATAC53900.067.269935
CGTATAG66100.067.190855
ATTATAG92150.067.186575
GTATACC42200.067.17736
Produced by FastQC (version 0.11.5)