FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_root_plusN_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_root_plusN_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3985886
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA72370.1815656544115913No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC57460.1441586638453784No Hit
GTTTTCTATATCGCCTGCGTAGTGATTACGTGGCTGGTATATGGCCGTAA50150.12581895217274153No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA46030.11548247993043453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG64600.067.832366
GTATAGC57450.067.258036
GCTATAC41450.067.213055
TTATAGC63850.067.2039266
CTATAGG67200.067.187046
CTTATAC45100.067.0505455
CTTATAG54300.067.034545
CTATACG44800.067.030796
CTATACC31500.066.999546
GGTATAC55750.066.797755
CGTATAA48800.066.700365
CCTATAC29400.066.6662145
GTATACG62050.066.445976
GTATAAG76500.066.430926
AGTATAC46700.066.4021155
CATATAG63150.066.286165
GCTATAG58400.066.28385
CGTATAG53300.066.2565845
ATTATAG81800.066.234275
GTTATAC48800.066.198325
Produced by FastQC (version 0.11.5)