FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_root_minusN_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_root_minusN_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4165673
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA79250.19024536971576983No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA67670.16244674029862644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG59800.068.3015755
GTATAGC62100.068.1396
TTATACG58850.067.9768146
CTATACG46150.067.87546
CCTATAC34400.067.862285
GGTATAC61150.067.652225
CTATACC36350.067.591766
GTATACG62650.067.484956
TTATAAC54550.067.368296
CTTATAG61400.067.319765
CGTATAA53200.067.2357565
GCTATAC50250.067.2128455
GTATAAG80150.067.1604846
TTATAGC65400.067.109016
TTATAGG130150.067.0945746
ATTATAG88850.067.005115
GTATAGG131300.066.9600756
TCTATAG64450.066.9034965
CTATAAG52000.066.835466
AGTATAC49350.066.8074345
Produced by FastQC (version 0.11.5)