FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_root_minusN_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_root_minusN_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4010052
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA74760.18643149764641456No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA62830.156681259993636No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC44960.11211824684567682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG61550.068.0652546
CTATAGG78150.067.8937156
CGTATAG54000.067.7949455
CCTATAC31550.067.558085
GGTATAC57750.067.392595
GTATAGC61050.067.3041466
GTATAGG132050.067.162616
CTTATAG54000.067.1468055
CGTATAA51150.067.124765
CTTATAC49300.067.0879065
GTATAAG79900.067.063746
GCTATAG55600.067.040035
TTATAGC61350.066.9179846
GGTATAG101250.066.852985
CTATACG43400.066.7728656
GATATAC46350.066.751635
GTTATAC50600.066.678515
CTATACC34500.066.54946
ATTATAG85900.066.535345
GCTATAC45850.066.410895
Produced by FastQC (version 0.11.5)