FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_root_minusN_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_root_minusN_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4560761
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA86720.1901437062805966No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC80960.1775142350147267No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA51430.11276626861175142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC35100.067.80585
CGTATAG62750.067.489575
GGTATAC67300.067.399245
CTTATAC50550.067.368485
CTTATAG60700.067.347155
TTATAGC68200.067.125646
GATATAC56050.067.002225
TTATACG68950.066.903096
GCTATAG64400.066.901725
GCTATAC47300.066.817725
CTATAAG52950.066.6945346
GTATAGC64650.066.588876
CTATACG51700.066.546936
CGTATAA55400.066.524815
GTATAGG140650.066.515726
CTATAGG77450.066.474696
CATATAG73300.066.466125
GGTATAG107500.066.418155
AATATAC84500.066.2717365
AGTATAC56200.066.2006155
Produced by FastQC (version 0.11.5)