FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_rootEV_plusN_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_rootEV_plusN_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3430020
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC332450.9692363309834927No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA85510.2492988379076507No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA56700.16530515857050396No Hit
CCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTT48920.1426230750841103No Hit
CTCAACTATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGA36820.10734631284948774No Hit
TGTTACTAATAGGACCCCGCTGGCACCTTATGAGAAATCAAAGTCTTTGG36250.10568451495909645No Hit
CTCAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGA35220.1026816170168104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG38250.068.627026
CTATACC38000.068.157476
TTATAGG88600.067.708386
CTTATAG48500.067.257315
CTATAGG53250.067.239026
CCTATAC28700.066.828855
GTATAGG93750.066.714256
CGTATAG49200.066.5849465
CGTATAA42950.066.5769965
TTATACC45600.066.468886
AATATAC55100.066.442425
TCTATAG55550.066.4082265
CTTATAC44550.066.385675
CTATAGC33100.066.2986766
GCTATAC36750.066.28535
TATACCG93850.066.195647
TTATAGC45050.066.192716
GGTATAG73550.066.097485
GGTATAA54750.065.9082645
GTATACC45450.065.764176
Produced by FastQC (version 0.11.5)