FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_rootEV_plusN_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_rootEV_plusN_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751281
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA120150.436705665470012No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA33100.12030759489852182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAC36700.067.328065
CTATACG23650.067.038536
GTATAGG47950.066.786696
CTATAGG34400.066.6408166
CTATACC26150.066.5184566
CCTATAC24650.066.4486855
GGTATAA54300.066.130985
CTTATAC37800.066.10955
CATATAG38400.066.079125
CTATAGC31100.066.059486
TCTATAG38300.065.97755
TTATAGG50200.065.884856
CTATAAC30600.065.8807456
CGTATAG36150.065.835185
CGTATAA50250.065.819295
GATATAC29150.065.7965
TTATAGC35750.065.7886056
CCTATAG24050.065.778025
CCTATAA39400.065.7344365
TTATACC29450.065.719966
Produced by FastQC (version 0.11.5)