FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_rootEV_plusN_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_rootEV_plusN_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4237292
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC247980.5852322662681732No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA105610.24923937269369212No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC80000.18879982781455704TruSeq Adapter, Index 2 (100% over 50bp)
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA54640.12895028239734246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG41900.068.243116
CCTATAC33650.067.917055
TTATAGG116100.067.284146
CTATACC45850.067.249246
CTATAAC43600.066.9468846
CTTATAG58500.066.886235
CTATAGG67450.066.52076
CGTATAG60600.066.4165045
GTATAGG121250.066.244796
GGTATAG99350.066.15745
GCTATAG60250.066.1052555
GCTATAC42550.065.966835
CCTATAA45850.065.951585
GGTATAC59650.065.890085
TTATACC56250.065.7662666
GGTATAA73800.065.586825
CGTATAA51550.065.516315
TATAGGG155300.065.399847
GTATACG58850.065.358496
CTATAAG42900.065.265476
Produced by FastQC (version 0.11.5)