FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_rootEV_minusN_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_rootEV_minusN_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331764
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC152340.45723526636340395No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA76450.22945802883997785No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA76260.22888776035757635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG32750.067.540636
CTATACC30550.067.134496
TTATAGG84700.067.0234456
GCTATAC37400.066.723265
CGTATAG47200.066.513495
CTTATAG43500.066.377975
TTATACC38250.066.338546
CTATAGG46800.066.334136
CCTATAC25000.066.078675
CGTATAA45450.066.071285
GTATAGG91750.065.993226
GGTATAG73550.065.5255365
TTATAGC43650.065.428246
CCTATAA35750.065.3972855
CTATAAC30250.065.370586
CGTATAC36000.065.3320165
CTTATAC40300.065.222015
GTTATAC45600.065.163165
GTTATAG72850.065.146255
TTATAAG55850.065.1105966
Produced by FastQC (version 0.11.5)