FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_rootEV_minusN_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_rootEV_minusN_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4279182
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC276230.6455205691181165No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA112920.2638822092633592No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA73670.17215907152348275No Hit
CCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTT45400.10609504339848129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG47800.068.606766
GCTATAC45100.068.2130665
CTATACC48750.068.0595256
CCTATAC36050.067.8620765
CGTATAG65000.067.7903065
TTATAGG115450.067.3908166
TTATAGC54750.066.9934546
CGTATAA55450.066.905155
CTATAGG65500.066.898796
TTATACC57200.066.755026
CTATAAC46050.066.729846
CTTATAG57200.066.693835
GTATAGC58050.066.5010456
TTATAAG66400.066.4137046
ATTATAG92600.066.4073265
CTTATAC53350.066.3897865
CTATAAG48150.066.14556
TATACGG77250.066.101617
GTTATAC58000.066.075635
CGTATAC48650.066.0412145
Produced by FastQC (version 0.11.5)